AI technology in sustainable HR practice provides competitive advantage to the firm by creating powerful synergy between the used resources and company goal with automatic task process. The process of data identification and analysis using AI replaces human intervention and includes AI supporting environmental and social governance based on data analysis and tracking processes. Along with the significant growth of women entrepreneurship in Assam, the demand for AI-driven services is also expanding in Assam based on the changing dynamics of Industry 4.0. Regarding this, the use of AI-powered tools and software, as Chabot’s, CRM and other HR practices provides agility to predict the customers’ needs and focus on the areas of improvement on an urgent basis. This research has evaluated the significance of AI in sustainable HR practices and the ratio of women entrepreneurs prefer to adopt this technology in their new business plan. Addressing the issues of women entrepreneurs to gather funds, social support and technological knowledge for AI implementation enriched this research to suggest relevant actions in improving such conditions and support sustainable business growth. A quantitative primary method in this research has included personal experiences of women entrepreneurs and associated people in AI implementation and developing sustainable HR practices. Hence, a critical finding on Assam’s scope in AI development and supporting the growth of women entrepreneurs can leverage the sustainable business plan by reducing human intervention and developing automated task process.