Ecotourism really can make a difference. It’s good for the environment, it supports conservation, and it preserves culture and history. For those living in poor countries, ecotourism can offer a path out of poverty. Majuli is a very different place with unique natural beauty and traditions. This study aims to evaluate the impact of ecotourism on society and economy from the perspectives of local people. To assess the effects of ecotourism 200 respondents were selected as sample from Majuli district Island. One-Sample Wilcoxon Signed-Rank Test were used. The results suggest that ecotourism has positive socio-economic impact on the Majuli district Island. The present study would reflect the implications of ecotourism in Majuli Island, its possibilities to ensure the well-being of people, tourism industry and environment.