Bioactive extracts from different parts of Leea macrophylla. (Roxb.) member of Leeaceae family was investigated for antimicrobial properties. The perennial shrub has been utilized in floklore medicinal system. Phytochemical rich herb has shown significant therapeutic effect in treatment of ailments with explicit pharmacological response including anti-inflammatory, antilithotropic, hepatoprotective, neuroprotective and antidiarroheal as well. Phytochemical investigation revealed the high amount of primary and secondary metabolites enriching the nutritious and medicinal value of the herb in study.  Presence of bioactives led researchers to conduct preclinical studies on the Leea macrophylla for its pharmacological response against pathogenic micro-organisms responsible for major setbacks in human health. The method implemented was well diffusion method for testing antimicrobial activity   against gram-positive, gram-negative pathogenic bacterial strains isolated clinically and certain infective fungal strains. Medicinally important and bioactive rich medicinal plant Leea macrophyllatested for antimicrobial studies. The experimental study was carried out for antimicrobial efficacy in aerial parts, fruitning bodies, underground parts as well as invitro grown callus for comparative analysis. The observation and interpretation of the experiment conducted were discussed in the presented literature. The findings in the current analysis supports and clear recommend that Ethanolic extracts possess significant antimicrobial property against microbial clinical isolates in study.