Aquatic medicinal plants play a crucial role in maintaining ecosystem balance and providing valuable resources for traditional medicine. However, these plants face numerous conservation challenges due to anthropogenic pressures and environmental changes. This research paper focuses on the conservation challenges and innovative approaches for protecting aquatic medicinal plants in Karauli District, Rajasthan. The study employs a mixed-methods approach, combining field surveys, interviews with local communities and stakeholders, and geospatial analysis to assess the current status of aquatic medicinal plants, identify key threats, and propose innovative conservation strategies. The field surveys revealed a total of 34 species of aquatic medicinal plants belonging to 21 families and 3 classes (Monocotyledons, Dicotyledons, and Pteridophytes). Some notable species include Bacopa monnieri (Brahmi), Centella asiatica (Gotu kola), Nymphaea nouchali (Blue lotus), and Acorus calamus (Sweet flag). The findings highlight the need for community participation, sustainable harvesting practices, and the integration of traditional knowledge with modern conservation techniques. The paper concludes by emphasizing the importance of collaborative efforts among government agencies, NGOs, and local communities in implementing effective conservation measures to safeguard the aquatic medicinal plant diversity in Karauli District.