The increasing global need for food has resulted in the heavy application of chemical fertilizers, which, although enhancing crop production, have led to significant environmental harm and diminished soil fertility over time. Biofertilizers—microbial agents that improve soil fertility via natural nitrogen fixation, phosphate solubilization, and growth-promoting compounds—provide a viable option for sustainable farming. This research examines the effects of biofertilizers on agricultural yield, soil quality, and ecological sustainability. Using field trials, lab analyses, and farmer surveys, the study seeks to assess the efficacy of biofertilizers in relation to traditional chemical inputs. The results are anticipated to offer proof that incorporates biofertilizers into conventional farming methods for attaining enduring agricultural sustainability.