Transport plays a vital role in economic development by facilitating the movement of people and goods; however, employees in this sector are increasingly exposed to significant levels of occupational stress due to demanding working conditions. This study examines the factors influencing work stress among employees of the Andhra Pradesh State Road Transport Corporation (APSRTC) in the Visakhapatnam Zone, analyzes its impact on employee well-being and job performance, and suggests suitable stress management measures. The study is based on primary data collected from 137 respondents, including drivers and conductors, using a structured questionnaire. The findings reveal that work stress among transport employees is moderate and primarily influenced by job-related, organizational, environmental, and personal factors such as long and irregular working hours, heavy workload, traffic congestion, inadequate rest, and limited organizational support. Statistical analysis shows that demographic variables such as age, education, salary, designation, and residence do not significantly influence stress levels. The study also identifies major consequences of work stress, including work–life imbalance, emotional instability, decreased work efficiency, reduced concentration, absenteeism, and increased accident risk. The study concludes that work stress has a significant negative impact on both employee well-being and organizational effectiveness, highlighting the need for improved working conditions, employee welfare measures, and structured stress management programs to enhance productivity and service quality in the transport sector.