Work-life balance has emerged as a critical factor influencing employee satisfaction, productivity, and long-term organizational sustainability, particularly within the rapidly evolving Information Technology (IT) sector. This study investigates the relationship between work-life balance practices and employee retention through a comparative analysis of Infosys, Wipro, Tata Consultancy Services, and IBM, with special reference to Gujarat. The research evaluates how organizational initiatives such as flexible working arrangements, remote work facilities, leave policies, and employee support mechanisms influence employees’ intentions to remain with their organizations. The study adopts a quantitative research approach and collects primary data from 200 IT employees using structured questionnaires. Statistical techniques, including Pearson correlation and regression analysis, were applied to examine the relationship between work-life balance and employee retention. The findings reveal a strong positive association between supportive work-life balance policies and employee retention levels. The regression analysis further indicates that effective work-life balance practices significantly contribute to employees’ commitment and reduce turnover intentions. The demographic analysis also suggests that employees across different age groups, educational backgrounds, and experience levels consider work-life balance an important aspect of their professional lives. The study concludes that organizations implementing employee-centric work-life balance strategies are more likely to achieve higher job satisfaction, improved workforce stability, and enhanced organizational performance. The research provides practical insights for IT companies in designing sustainable HR policies that support employee wellbeing and strengthen retention strategies.